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In Mar chiquita coastal lagoon (Bs. As.) zinc (Zn) levels up to 1224ug/L has been detected. The aim of this work was to study the effects of Zn exposure on Na+K+ATPase activity (NKA) in anterior and posterior gills (AG, PG) and maltase (Mal) and sucrase (Suc) activity in hepatopancreas (H) of the euryhaline crab N. granulata. Adult males acclimated for 10 days in 35 (osmoconformation) and 10 (hyperregulation) salinity (S) were exposed for 96 h in the absence or presence of Zn 1224ug/L. The supernatant (10000xg) from AG and PG (0.25 M sucrose /EGTA Tris pH7.4) and H (Tris- HCl 0.1M pH7.4) homogenates were used. NKA was determined by measuring ATP hydrolysis in the presence of (mM): 20 Imidazole (pH 7.4)/ 100 NaCl/ 30KCl/ 0.5 EGTA (Control: without KCl, with 1mM ouabain). Mal and Suc (ìgglucose x min-1 x mgprotein- 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. Adult males acclimated for 10 days in 35 (osmoconformation) and 10 (hyperregulation) salinity (S) were exposed for 96 h in the absence or presence of Zn 1224ug/L. The supernatant (10000xg) from AG and PG (0.25 M sucrose /EGTA Tris pH7.4) and H (Tris- HCl 0.1M pH7.4) homogenates were used. NKA was determined by measuring ATP hydrolysis in the presence of (mM): 20 Imidazole (pH 7.4)/ 100 NaCl/ 30KCl/ 0.5 EGTA (Control: without KCl, with 1mM ouabain). Mal and Suc (ìgglucose x min-1 x mgprotein- 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. and posterior gills (AG, PG) and maltase (Mal) and sucrase (Suc) activity in hepatopancreas (H) of the euryhaline crab N. granulata. Adult males acclimated for 10 days in 35 (osmoconformation) and 10 (hyperregulation) salinity (S) were exposed for 96 h in the absence or presence of Zn 1224ug/L. The supernatant (10000xg) from AG and PG (0.25 M sucrose /EGTA Tris pH7.4) and H (Tris- HCl 0.1M pH7.4) homogenates were used. NKA was determined by measuring ATP hydrolysis in the presence of (mM): 20 Imidazole (pH 7.4)/ 100 NaCl/ 30KCl/ 0.5 EGTA (Control: without KCl, with 1mM ouabain). Mal and Suc (ìgglucose x min-1 x mgprotein- 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. Adult males acclimated for 10 days in 35 (osmoconformation) and 10 (hyperregulation) salinity (S) were exposed for 96 h in the absence or presence of Zn 1224ug/L. The supernatant (10000xg) from AG and PG (0.25 M sucrose /EGTA Tris pH7.4) and H (Tris- HCl 0.1M pH7.4) homogenates were used. NKA was determined by measuring ATP hydrolysis in the presence of (mM): 20 Imidazole (pH 7.4)/ 100 NaCl/ 30KCl/ 0.5 EGTA (Control: without KCl, with 1mM ouabain). Mal and Suc (ìgglucose x min-1 x mgprotein- 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. +K+ATPase activity (NKA) in anterior and posterior gills (AG, PG) and maltase (Mal) and sucrase (Suc) activity in hepatopancreas (H) of the euryhaline crab N. granulata. Adult males acclimated for 10 days in 35 (osmoconformation) and 10 (hyperregulation) salinity (S) were exposed for 96 h in the absence or presence of Zn 1224ug/L. The supernatant (10000xg) from AG and PG (0.25 M sucrose /EGTA Tris pH7.4) and H (Tris- HCl 0.1M pH7.4) homogenates were used. NKA was determined by measuring ATP hydrolysis in the presence of (mM): 20 Imidazole (pH 7.4)/ 100 NaCl/ 30KCl/ 0.5 EGTA (Control: without KCl, with 1mM ouabain). Mal and Suc (ìgglucose x min-1 x mgprotein- 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. Adult males acclimated for 10 days in 35 (osmoconformation) and 10 (hyperregulation) salinity (S) were exposed for 96 h in the absence or presence of Zn 1224ug/L. The supernatant (10000xg) from AG and PG (0.25 M sucrose /EGTA Tris pH7.4) and H (Tris- HCl 0.1M pH7.4) homogenates were used. NKA was determined by measuring ATP hydrolysis in the presence of (mM): 20 Imidazole (pH 7.4)/ 100 NaCl/ 30KCl/ 0.5 EGTA (Control: without KCl, with 1mM ouabain). Mal and Suc (ìgglucose x min-1 x mgprotein- 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. N. granulata. Adult males acclimated for 10 days in 35 (osmoconformation) and 10 (hyperregulation) salinity (S) were exposed for 96 h in the absence or presence of Zn 1224ug/L. The supernatant (10000xg) from AG and PG (0.25 M sucrose /EGTA Tris pH7.4) and H (Tris- HCl 0.1M pH7.4) homogenates were used. NKA was determined by measuring ATP hydrolysis in the presence of (mM): 20 Imidazole (pH 7.4)/ 100 NaCl/ 30KCl/ 0.5 EGTA (Control: without KCl, with 1mM ouabain). Mal and Suc (ìgglucose x min-1 x mgprotein- 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. ìgglucose x min-1 x mgprotein- 1) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity. ) was assayed by hydrolysis of maltose or sucrose in 0.1 M maleate/ OHNa. In the absence of Zn, NAK in BP was higher in 10 S (t-test, p=0.03); in the presence of Zn was similar in 35 and 10 S (p>0.05). NAK in BA in the absence of Zn was similar in both S (p>0.05); in the presence of Zn was higher in 10 S (t-test, p=0.04). Mal and Suc both in the absence and the presence of Zn were similar in both S (p>0.05). The response of NAK in gills to Zn and the maintenance of Mal and Suc in hepatopancreas suggest a differential effect of Zn at the biochemical-physiological level and in relation to salinity.

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