EFFECT OF CADMIUM STRESS ON TRANSGENIC AND NON-TRANSGENICSOYBEAN PLANTS (Glyicine max. L) (molecular analysis)

GOMEZ MANSUR NABILA MARIA; PEREZ CHACA MARIA VERÓNICA; MOLINA ALICIA SUSANA; ZIRULNIK FANNY

Tucumán

Congreso; III Reunión Conjunta de Sociedades de Biología de la República Argentina; 2015

In Argentina, 90% of soybeans are resistant to glyphosate, RR (Roundup Ready) transgenic varieties. It is an interesting challenge to find advantageous properties of non-transgenic species (NT) respect transgenic plants (T RR) against various stressors, in an attempt to use native species before the genetically transformed ones. Cadmium (Cd) is a contaminant that interferes with the uptake, transport and use of essential elements (Ca, Mg, P and K) and water, causing oxidative stress. The objective of this study is to determine the different biochemical behavior of T and NT soybean, under Cd oxidative stress. Soybean leaves NT and T with Cd 40 M contamination for 24 hours was the experimental model. Total RNA extraction was performed by phenolic-trizol method and RT-PCR and the genes expression of catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) were measured as antioxidant enzimes. The results showed CAT expression significantly higher in NTCd respect TCd. APX showed no significant differences between T vs NT Cd treated plants. GR expression increases significantly in presence of Cd compared to controls without Cd (Co) in both T and NT, showing higher expression in TCd that NTCd. GR basal state transcription (Co 0 hs) of NT variety is markedly higher compared to the T ones, which may be advantageous in relation to the antioxidant defense. There are differences in the mechanisms of response to oxidative stress and antioxidant defense by molecular changes between transgenic DM 4800 (RR) and non-transgenic DM 48 soybeans, when they undergo stress by Cd.