Vascular calcification in an experimental type 1 Diabetes mellitus model: benefit of naringin treatment

RIVOIRA MA; MASSHEIMER V; RODRIGUEZ VA; RAUSCHEMBERGER MB; TOLOSA DE TALAMONI NG

Buenos Aires

Otro; XXXVI Reunion anual AAOMM 2019; 2019

Vascular calcification (VC) is an important complication of type 1 Diabetes mellitus (DM). Several studies suggest that the antioxidant naringin (NAR) is beneficial for treatment of DM, but its effect on the VC has not been investigated. The aim of this work was to know whether NAR could attenuate the VC in Wistar male rats with DM. Three groups of animals were used: controls, diabetic rats (treated with 60 mg streptozotocin /kg b.w.: STZ), diabetic rats treated with NAR (40 mg/kg b.w.). After 30 days of treatment, plasma was withdrawn and rats were sacrificed to obtain the aortas. Endothelial cells (EC) from aortas were cultured and NO? was measured by the Griess´s method. ANOVA and Bonferroni test were used for statistical analysis. NO? production was reduced in STZ rats, which was highly blocked by NAR. In control aortas, estrone (E1) and genistein (Gen) stimulate NO? but in aortas from STZ rats there was lack of NO? stimulation by those hormones. However, NAR restored the capability to stimulate NO? production under E1 and Gen treatments. Isolated aortas from the different groups of animals were exposed to a pro-calcific medium with glycerophosphate for 7 days; the aortas were decalcified and the released calcium was measured by a commercial kit. Calcium content from aortas of STZ rats was 74% higher than that from the control rats. NAR treatment reduced calcium incorporation in aortas from STZ rats to values closed to the control ones. These data were confirmed by AgNO3 staining. Aortas from STZ rats showed multiple sites of calcification, effect that was abolished by NAR treatment. All data suggest that NAR could prevent damage of the vascular morphology and functionality in DM.