ACUTE TOXICITY AND ANTI-ULCEROGENIC ACTIVITY OF INFUSION OF Vaccinium myrtillus L. STEM at 5%

GENTA S. B.; SERRA BAECELLONA, C; HABIB N. C.; ALMIRÓN, C

Tafí Viejo, Tucumán

Jornada; XXXVI Jornadas Científicas de la Asociación de Biología de Tucumán; 2019

Asociación de Biología de Tucumán

Gastric ulcer is a gastrointestinal disease with high prevalence worldwide. Our research group studies the possible gastroprotective effectsof natural compounds extracted from Vaccinium myrtillus (VM) stems, an agricultural solid waste with potential bioactivity. The aim ofthis work was to study the possible mechanisms of action involved in the gastroprotective effect of the infusion at 5% (I) of VM stems inan ethanol-induced gastric ulcer model in Wistar rats. The toxicity profile of the aqueous extract of VM stems was also established bybioassays with Artemia salina and by acute toxicity tests in rats. The phytochemical characterization of the I of VM stems demonstrated thepresence of flavonoids, phenols, and tannins as major components. The oral administration of I?VM stems at different doses (50?150mg/kg) produces a significant reduction in the severity and percentage of ulceration, maintaining the normal histological structure of thestomach. These effects are linked to the production of mucus as a protective element. The pre-treatment with I?VM stems presented aneffective antioxidant effect and a lipid peroxidation inhibition capacity. The use of a model with both, a nitric oxide (NO) synthaseinhibitor and a NO precursor suggests the indirect participation of NO in the gastroprotective effect of the extract. No toxicity signs wereobserved in both, A. salina and rats at doses assays. The findings presented in this work allow us to postulate that this extract of simpleelaboration presents an effective anti-ulcerogenic activity without adverse effects.