Sub-anorexigenic doses of leptin activates the cardiac leptin-TRH hypertrophic pathway

MAIA AISICOVICH; ROSATI MACARENA; PERES DIAZ LUDMILA; SILVIA I. GARCÍA; MARIANO L. SCHUMAN; MARÍA S. LANDA

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina Invesigación Clinica (SAIC); 2019

Sociedad argentina de Investigacion Clinica (SAIC)

Cardiac TRH (cTRH) induce left ventricular hypertrophy (LVH) and fibrosis. Additionally Leptine (Lep) induces TRH, until now only described in CNS.Our previous results on obese mouse models suggest that high Lep levels may impact cardiac tissue inducing cTRH expression and consequently stimulate LVH mediated by cTRH. So far this novel Lep-cTRH pathway has not been described in lean mice. We used C57 adult male mice to evaluate if sub-anorexigenic Lep doses could stimulate cTRH system hypothesizing that low doses of Lep could activate the Lep -cTRH pathway. For 3 weeks mice received subcutaneous leptin (10ug/kg/day) or saline, with or without cTRH blockage by intracardiac injection of siRNA-TRH or siRNA-control (n=7). As expected, Lep did not modify body weight, food intake or blood pressure during all the experiment, as there were no differences between those treated with Lep vs saline confirming the sub-anorexigenic Lep dose.As hypothesized we found that Lep significantly increased (p