Megakaryocyte (MK)-stromal cell interactions: effect on proliferation, proplatelet production, and survival

MARTA RF; LEV PR; DISCIANNI LUPI AD; HELLER PG; GOETTE NP; RUBIO MF

Mar del Plata, Buenos Aires

Congreso; LXIV Reunion Anual dela Sociedad Argentina de Investigacion Clinica; 2019

Sociedad Argentina de Investigacion Clinica

Bone marrow stroma cells (SC) provide a proper environment for the development of hematologic lineages. They contribute to the regulation of haematopoietic stem cells growth and differentiation by producing and releasing cytokines, growth factors and extracellular matrix proteins. In order to study in vitro megakaryopoiesis and thrombopoiesis, the incorporation of the different SC would be an attractive model. Our objective was to evaluate the participation of different types of SC on megakaryopoiesis, thrombopoiesis and MK survival under in vitro culture conditions. CD34-positive progenitors obtained from umbilical cord blood were cultured in medium supplemented with thrombopoietin, with or without umbilical cord endothelial cells (HUVEC), bone marrow mesenchymal stem cells (MSC), skin fibroblasts (SF) (all human) or mouse fibroblast cell line (L929). Biological samples were obtained after informed consent and Ethics Commitee approval. The number of MK (CD61-positive cells) and mature MK (CD42-positive cells) were increased in the presence of MSC and HUVEC, reaching statistical significance in the latter (CD61, p=0.016). In the presence of L929, the number of total and mature MKs decreased (p