CROSSTALK BETWEEN ADIPOCYTES AND BREAST CANCER CELLS: MODULATION OF NUCLEAR RECEPTOR COACTIVATOR 3 (RAC3) IN FAT CELLS

LIRA, M C; ROSA, F D; SOARES MACHADO M; PALMA A; MARINO, GABRIELA; COSTAS M A; RUBIO M F

Mar Del Plata

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2019; 2019

Sociedad Argentina de Investigación Clinica

Adipocytes account for the largestproportion among the cells that comprise breast tissue. Althoughthey are considered to be a critical cell type in the tumormicroenvironment of breast cancer, there is still unclear themolecular mechanisms that control their behavior in this context.We have demonstrated that the transcriptional coactivator RAC3decreases during adipogenesis and its high expression in adiposetissue adjacent to breast tumors correlate with different markersof tumoral progression in patient samples. Therefore, the aim ofthis work was to further study the role of this molecule in an invitro model. Conditioned media (CM) were collected from nontumoralor tumoral breast human cell lines with the followingRAC3 mRNA levels: MCF10A: 1.55 ± 0.73, MDA-MB-231: 167.20± 6.91, T47D: 571.00 ± 12.59. 3T3-L1 derived-murineadipocytes were stimulated with CM or serum-free medium.RAC3 expression levels measured by qPCR resulted significantlyhigher in adipocytes stimulated with CM from high RAC3 levelsexpressingT47D cells (9.25 ± 1.02) compared to the otherconditions: basal (1.00 ± 0.00), MCF10A (2.33 ± 0.96), MDAMB-231 (2.80 ± 1.19) (p