BECAS
GARCES Mariana Soledad
congresos y reuniones científicas
Título:
NO AND INFLAMMATION AS EFFECTORS OF CARDIAC MITOCHONDRIAL DYSFUNCTION AND ROS PRODUCTION IN LOW-GRADE AND SEVERE ENDOTOXEMIA.
Autor/es:
VICO TAMARA; VIRGINIA VANASCO; MARCHINI TIMOTEO; ADAN AREAN JUAN SANTIAGO; CALABRÓ VALERIA; GARCES MARIANA; CATUOGNO FRANCO; LOREZENTTI MARIO; FERRERO MARIANA; GONZALEZ MAGLIO DANIEL; BALDI PABLO; EVELSON PABLO; ALVAREZ SILVIA
Reunión:
Congreso; LXII Reunión Científica Anual de Sociedad Argentina de Investigación Clinica (SAIC); 2017
Resumen:
Myocardial dysfunction is a complication of severe sepsis and endotoxemia. Previously, we described cardiac mitochondrial dysfunction in this syndrome. Mechanisms of how systemic inflammation could damage heart function remains still unknown. Cytokines, NO and bioenergetics derangements are thought to be involved. The aim was to analyse systemic NO and ROS in a model of low-grade and severe endotoxemia. Female Sprague-Dawley rats (55 days) were injected i.p. with a single dose of LPS 0.5 mg/kg, 8 mg/kg or vehicle (control), 6h later animals were sacrificed. White blood cells (WBC) and differential count were analysed. DFC-DA and DAF-2-DA were used to estimate intracellular ROS and NO concentration by polymorphonuclear cells (PMN), respectively. Nitrite/nitrate content and proinflammatory cytokines levels (TNFα and IL-6) were analysed in plasma samples. Mitochondrial H2O2 production was measured using Amplex Red probe. WBC count showed difference between groups: control 2638 cells/mm3 (N:17%/L:77%/M:5%/E:1%/B:0%), LPS 0.5: 6771 cells/mm3 (N:61%/L:28%/M:7%/E:0%/B:4%) and LPS 8: 1021 cells/mm3 (N:58%/L:40%/M:2%/E:0%/B:0%). PMN cells from LPS-treated animals increased intracellular NO by 147% (LPS 0.5) and 164% (LPS 8) compared with control (p