THE ROLE OF MITOCHONDRIA IN THE OXIDATIVE RESPONSE OF RAW 264.7 MURINE MACROPHAGES EXPOSED TO AIR POLLUTION PARTICULATE MATTER

CACERES LOURDES; MARIELA PAZ; ADAMI PAMELA; OJEDA DIEGO; GARCES MARIANA; CALABRÓ VALERIA; TASAT DÉBORAH; MORELLI LAURA; ALVAREZ SILVIA; GONZALEZ MAGLIO DANIEL; MARCHINI TIMOTEO; EVELSON PABLO

Congreso; LXII Reunión Científica Anual de Sociedad Argentina de Investigación Clinica (SAIC); 2017

The exposure to environmental particulate matter (PM) is associated with pulmonary oxidative stress and inflammation. Alveolar macrophages are suggested to play a central role in this scenario, since they produce inflammatory mediators and reactive oxygen species. The aim of this work was to study the redox status and the role of mitochondria in macrophages after the exposure to Residual Oil Fly Ash (ROFA), a PM surrogate rich in transition metals. The murine cell line RAW 264.7 was exposed to ROFA at 100 μg/mL for 24 hs. Cell viability was not significantly affected under these experimental conditions. Intracellular redox status was assessed by flow cytometry as dichlorofluorescein mean fluorescence intensity (MFI), which was increased by 30% following ROFA incubation compared to the control group (p