Assesmemt of the potential health impact of silver nanoparticles exposure

GARCES MARIANA; CALABRÓ VALERIA; MARCHINI TIMOTEO; GUAGLIANONE ALEJANDRO; CACERES LOURDES; VICO TAMARA; GALDOPORPORA JUAN; MORETTON MARCELA; DESIMONE MARTÍN; ALVAREZ SILVIA; EVELSON PABLO

Congreso; LXII Reunión Científica Anual de Sociedad Argentina de Investigación Clinica (SAIC); 2017

Silver nanoparticles (AgNP) are commonly used in different commercialized products, such as disinfectant sprays, personal hygiene products, and several biomedical applications. Along with the development of AgNP, the question of their possible toxicity has increasingly gained attention. Since oxidative stress has been postulated to play a major role in AgNP toxicity, the aim of this work was to evaluate the oxygen metabolism in the lung after AgNP inhalation. Characterization of AgNP by TEM showed a spherical shape and the absence of NP aggregation. Hydrodynamic diameter and zeta potential, as assessed by dynamic light scattering, was 17 ± 6 nm and -40 ± 7 mV respectively. Desorption studies showed a 24% release of Ag from AgNP after 1 h of incubation. The ability of AgNP to participate in Fenton chemical reaction was also evaluated, showing that AgNP was able to induce the production of reactive oxygen species in a concentration dependent manner. For in vivo studies, female Balb/c mice (25 g) were intranasally instilled with a AgNP suspension (0.1 mg Ag/kg body weight). The control group was treated with saline solution. Samples were collected after 1 h. Biodistribution studies showed that the main organ of Ag deposition was the lung (450 ± 50 ppb Ag/g tissue). In lung tissue, the O2 consumption increased by 31% (control group: 300 ± 20 ng-at O/min g tissue, p