Mitochondrial function in the brain cortex of rats in an experimental glaucoma model

AILEN GALA HVOZDA ARANA; ROMINA LASAGNI VITAR; CLAUDIA REIDES; CALABRÓ, VALERIA; GARCES MARIANA; FABIÁN LERNER; EVELSON PABLO; SUSANA LLESUY; SANDRA FERREIRA

Congreso; LXIII Reunión Anual de la SAIC; 2018

Glaucoma is the second leading cause forblindness worldwide and damages structures in the brain,including the primary visual cortex. The aim was to evaluate thealterations of mitochondrial function in an experimentalglaucoma model. Three-month female Wistar rats were divided intwo groups (n= 8): glaucoma (GG) in which rats were operatedunder a microscope by cauterized two of the episcleral veins andcontrol (CG) which received a sham procedure. Seven days aftersurgery rats were euthanized, brain cortex was separated, andmitochondria were isolated (CICUAL FFyB n° 3314). Thefollowing markers were evaluated: oxygen consumption (OC),ATP production, hydrogen peroxide (H2O2) and superoxide anion(O2-) production, the activities of superoxide dismutase (SOD),complex I-III and complex II-III, cardiolipin content,mitochondrial membrane potential, and NADPH oxidase-4(NOX4) expression. Comparing to CG, GG showed a decrease inATP production (23 %, p