TIME COURSE OF INFLAMMATORY AND OXIDATIVE RESPONSE IN ENVIRONMENTAL PARTICULATE MATTER EXPOSED MACROPHAGES

CACERES LOURDES; PAZ MARIELA; GARCES MARIANA; CALABRÓ VALERIA; MAGNANI NATALIA; GELPI RICARDO; CALTANA LAURA; TASAT DÉBORAH; SILVIA ALVAREZ; GONZALEZ MAGLIO DANIEL; MARCHINI TIMOTEO; EVELSON PABLO

Congreso; Reunión anual de sociedades de biociencia 2019; 2019

Alveolar macrophages are key pulmonary effectors which induce inflammation and oxidative stress after environmental particulate matter (PM) exposure. Therefore, it is of great interest to characterize the oxy-inflammation crosstalk in this scenario. The aim of our work was to study the time course of both oxidative and inflammatory response observed in macrophages after the exposure to Residual Oil Fly Ash (ROFA), a PM surrogate rich in transition metals. The murine cell line RAW 264.7 was exposed to ROFA at 100 μg/mL for 1, 3, 6, and 24 h. Cell viability was not significantly affected under these experimental conditions. Intracytoplasmic complexity was assessed by flow cytometric analysis of light scattering properties (SSC-H) as a cell activation indicator. We found an increase beginning at 1 h time point for the ROFA exposed group (1.00±0.01 vs 1.30±0.02 % control fold change, p