Sub-anorexigenic doses of leptin activates the leptin-TRH cardiac hypertrophic pathway.

AISICOVICH M; SCHUMAN ML; PERES DIAZ LS; ROSATI M; LANDA MS; GARCIA SI

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencia SAIC . SAFE . SAB . SAP AACYTAL . NANOMED-ar . HCS; 2019

Cardiac TRH (cTRH) induce left ventricular hypertrophy(LVH) and fibrosis. Additionally Leptine (Lep) induces TRH, until now only describedin CNS.Studying obese Agouti and ob/ob mice, we speculatethat high Lep levels may impact cardiac tissue inducing cTRH expression andconsequently stimulating LVH mediated by cTRH. So far this novel Lep-cTRHpathway has not been described in lean mice physiology.   We used C57 adultmale mice to evaluate if sub-anorexigenic Lep doses could stimulate  cTRH system hypothesizing that low doses of Lepcould activate the    Lep -cTRHpathway. For 3 weeks mice received leptin (10ug/kg/day) or saline, with or without cTRH blockage by intracardiac injection of siRNA-TRH or siRNA-control (n=7).  As expected, Lep did not modify body weight, food intake or blood pressure during all the experiment, as there were no differences between those treated with Lep vs saline confirming the sub-anorexigenic Lep dose.As hypothesized we found that Lep significantly increased (p