CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Peripheric Blood Mononuclear Cells (PBMC) and polymorphonuclear leukocytes (PMNL) upregulate Secretory Leukocyte Protease Inhibitor (SLPI) expression
Autor/es:
COSTA, M. JULIETA; TATEOSIAN, NANCY; AMIANO, NICOLÁS; REITERI, MACARENA; SÁNCHEZ, MERCEDES; ROMEO, HORACIO; CHULUYAN, H. EDUARDO
Lugar:
Buenos Aires, Argentina
Reunión:
Congreso; First French-Argentine Immunology Congress. LVIII Reunión Anual de la Sociedad Argentina de Inmunología; 2010
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
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SLPI is a serpin of 11,7 kDa that belongs to the family
of four disulfide core proteins characterized by whey acid protein motif. The gene encoding SLPI is modulated at
transcriptional and translational levels by pro-inflammatory cytokines.
However, there is no data if the SLPI expression can be modulated by leukocytes
recruited at the inflammation site. Therefore, the aim of this work was to
examine the modulation of the SLPI expression by leukocytes on type II-like
alveolar cell line (A549). SLPI was measured by sandwich ELISA in culture cells
supenantant of A549, PMNL (resting and LPS-activated), PBMC (resting and
IL-2-treated), lymphocyte (resting and IL-2-activated) or cocultures of leukocytes
with A549 cells. CS were harvested at 18 h of incubation. SLPI (ng/ml) was
present in the CS of A549 (0,06±0,02), PMNL (0,18±0,06), LPS activated-PMNL
(0,54±0,06), PBMC (0,1±0,05), but not in
IL-2-treated PBMC, lymphocytes or IL-2-treated lymphocytes. The co-culture of
A549 with PMNL or PBMC, but not with LPS-activated PMNL, IL-2-treated PBMC,
lymphocyte or IL-2-treated lymphocyte increased SLPI production (A549+PMNL:
0,84±0,21; A549+PBMC: 5,02±0,33,p<0.001 and p<0.001). In order to
determined whether the increased in SLPI concentration in the co-culture of
A549 with PMNL or PBMC were mediated by soluble factor released by leukocyte,
A549 cells were cultured with CS derived from PMNL or PBMC. When A549 cells
were treated with PMNL or PBMC-derived CS, the SLPI secretion was increased by
three fold (p<0.001 and p<0.001). Interestingly, the CS-derived from
activated-PMNL or IL-2-treated PBMC was not able to increase the SLPI
secretion. However, if activated PBMC were pre-treated with SLPI, the SLPI
secretion is restored. Overall these results demonstrate that epithelial cells
secretion of SLPI may be upregulated by factors released by neutrophils and
PBMC, but not lymphocytes or activated leukocytes.