Natriuretic peptide system in the adipose tissue: the effect of high fat diet.

MARTÍNEZ TAMBELLA JOAQUÍN; SORIA DAMIÁN; SARAVIA MELISA; MAGALDI ROCÍO; SOSA AGUSTINA; ROMERO MARIANA; ZAGO VALERIA; SCHREIER LAURA; TOMAT ANALÍA; ELESGARAY ROSANA; ARRANZ CRISTINA; CANIFFI CAROLINA

Rosario

Congreso; REUNIÓN ANUAL 2019. SOCIEDAD ARGENTINA DE FISIOLOGÍA; 2019

Obesity and overweight are risk factors for the development of cardiovascular diseases. Natriuretic peptides (NP) modulate cardiovascular homeostasis and have lipolytic effects on adipose tissue (AT). The bioavailability of these peptides depends on the neprilysin enzyme (NEP) which is responsible for the degradation of NP.Objective: To evaluate the expression of components responsible for the degradation of NP in retroperitoneal AT (rAT) of rats fed high-fat diet (HFD) for 11 weeks.Experimental design: Wistar rats received, from weaning until the 14th week of life, HFD (60% of total calories correspond to fat) or control diet (CD). At the end of the experimental period, body weight (BW) and systolic blood pressure (SBP) were measured, and oral glucose tolerance test (OGTT) was performed. The animals were sacrificed, and rAT morphology was evaluated byhematoxylin-eosin staining. NEP expression were evaluated (RT-qPCR) in rAT. Results are expressed as mean±SEM. Statistical analysis: Student test (n=6 rats/group; *p