IFNβ expression in the nervous and respiratory system of bovine alpha-herpesviruses-infected calves

BURUCÚA, MERCEDES; QUINTANA, SILVINA; ODEÓN, ANSELMO; PÉREZ, SANDRA; MARIN, MAIA

Mar del Plata

Encuentro; Encuentro SAIC-SAI-SAFIS; 2018

Sociedad Argentina de Inmunología/ Sociedad Argentina de Inmunología Clínica/ Sociedad Argentina de Fisiología

Induction of interferon (IFN) β is an important feature of bovine herpesviruses (BoHV)infections. The aim of this research was to determine the variations in IFNβ expressionin the respiratory tract and nervous system of calves during BoHV-1 and BoHV-5experimental acute infection [6 days post-infection (dpi), n=4] and reactivation(dexamethasone treatment, 25 dpi, n=4) in comparison to mock-infected animals (n=2).RNA was extracted from nervous system [olfactory, frontal and posterior cerebralcortex, cervical medulla and trigeminal ganglia (TG)] and respiratory tract(retropharyngeal lymph nodes, nasal mucosa, trachea and lung) to determinate the geneexpression of IFNβ1 by RT-qPCR. Relative expression analysis was performed withREST software (P