Hypervirulent Clostridioides difficile BI/NAP1/027 strain regulates T cell responses in an in vivo murine model

HERNANDEZ DEL PINO RODRIGO EMANUEL; CAJIAO MARIA FERNANDA; BARBERO ANGELA MARIA; CELANO JOSEFINA; ESTERMANN MARTIN; ESPAÑOL LAUREANO; MORRO LORENZO; MACHAIN MONICA; PASQUINELLI VIRGINIA

Tucuman

Congreso; LVII Reunión Anual de la Sociedad Argentina de Inmunología.; 2019

Clostridioides difficile, the etiologic agent of antibiotic-associated diarrhea, is a Gram-positive, anaerobic, spore-forming bacterium. BI/NAP1/027 is a hypervirulent strain that has dramatically changed the epidemiology of C. difficile infection (CDI). Since antibiotic runs the risk of prolonged gut microbiota perturbation and so recurrence of infection, identification of key effector molecules of host immune response against CDI may provide new therapeutic targets and biomarkers to improve treatment and diagnoses.We used a murine model to study the adaptive immune response during CDI. After induction of dysbiosis, mice were infected with spores from BI/NAP1/027 strain. Infected mice evidenced typical CDI symptoms such as diarrhea, hunched posture, and weight loss (p