Incidence of Sperm Surface Autoantibodies and Relationship with Routine Semen Parameters and Sperm Kinematics

VERÓN, GUSTAVO LUIS; MOLINA, ROSA ISABEL; TISSERA, ANDREA DANIELA; ESTOFAN, GUSTAVO MARTÍN; MARÍN-BRIGGILER, CLARA ISABEL; VAZQUEZ-LEVIN, MÓNICA HEBE

CABA

Congreso; Reunión Anual de la Sociedad Argentina de Andrología (SAA) 2016; 2016

Sociedad Argentina de Andrología

ProblemAntisperm antibodies (ASA) are associated with male subfertility. However,results on sperm surface autoantibodies are controversial, therelationship between ASA and semen parameters (WHO, 2010) isunknown, and data on ASA and sperm kinematics are scarce.Method of studyA retrospective study carried out in men undergoing routine semenanalysis (WHO 2010), ASA evaluation (direct SpermMARTM (IgG) test),and computer-assisted sperm analysis (CASA).ResultsA 2.6% and a 5.9% incidence of ASA-positive cases were found (cut-off50% and 10%, respectively; n = 7492). ASA-positive samples had lower(P < 0.0001) sperm concentration, count, motility, and hypo-osmoticswelling (HOS) test score. HOS results did not correlate with spermvitality in normozoospermic samples with high ASA levels. In unselectedsamples, ASA-positive samples (cut-off 50%) showed decreasedsperm kinematics (VSL, VAP, LIN, ALH, STR, BCF, WOB), but in normozoospermicsamples, ASA-positive and ASA-negative subgroups hadsimilar CASA results.ConclusionsASA evaluation is highly relevant in full semen assessment.