Prosopis strombulifera (Lam.) benth modulates immune cell activation

ABBA, ROMINA LOURDES; PERSIA, FABIO; CARGNELUTTI, DIEGO; HAPON, MARÍA BELÉN; MACKERN OBERTI, JUAN PABLO; GAMARRA LUQUES CARLOS

Mendoza

Otro; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2016

Sociedad de Biología de Cuyo

Autoimmune diseases are characterized by alterations affecting innate and adaptive immune cells. Although major progress has been made in elucidating the molecular mechanisms responsible for autoimmunity, current therapies for autoimmune disorders have not improved considerably. The aim of this work was to assess the immunosupressive effects of the native plant Prosopis strombulifera(Lam.) Benth (Ps) by in vitroassays. Splenocytes were obtained from C57BL/6 mice. Parameters of splenocytes and CD4+ T cell activation such as CD69 expression by FACS after concanavalin A (ConA) stimulation were used to evaluate the immune modulation of Psfrom cell cultures (24hs). IFN-ɤproduction after ConA stimulation was analyzed by ELISA. Interestingly, we found that Psdecreased the frequency of activated (CD69+) splenocytes after ConA stimulation comparedto control cultures (Ps: 30.91±1.32 vs control: 11.06 ± 0.38; p=0.0048). Similarly, Psimpaired CD4+ activation after ConA stimulation (Ps: 51.98 ± 1.94 vs control: 16.08 ± 0.03; p=0.0029). Although not statistically significant, when IFN-ɤproduction in response to ConA was evaluated, we found that splenocyte cultures in the presence of Psdisplayed lower levels than control cultures (Ps: 249.3 ± 92.25 vs control: 70.66 ± 7.85; p=0.0967). These data suggest that Psimpaired immune cell activation and reduced the production of the pro-inflammatory cytokine IFN-ɤwhich, in turn, may decrease immune responses. Although much work is needed to completely understand the underlying mechanisms of this immune suppression, our results highlight the immunotherapeuticpotential of Psin immune-mediated disorders