Cell-free DNA is elevated in chronic myeloproliferative neoplasms: correlation with thrombosis and disease progression

DE LUCA G; MARIN OYARZUN CP; GLEMBOTSKY AC; GUTIERREZ M; CASTRO RIOS MA; MOIRAGHI B; VICENTE A ; SACKMANN F; BENDEK G; VARELA A; CORTES GUERRIERI V; VERRI V; CAULA V; VALLEJOS V; LEV PR; GOETTE NP; AYALA D; MARTA RF; SCHATTNER M; HELLER PG

Congreso; ISTH 2020 Virtual Congress; 2020

Background: Thrombosis remains the main complication in myeloproliferative neoplasms (MPN). Although age, previous thrombosis and JAK2V617F are associated with thrombotic risk, additional predictive factors are lacking. Cell-free(cf) DNA is associated with thrombosis in diverse prothrombotic conditions and was found elevated in small MPN cohorts. Aims:To assess whether cfDNA is useful as a biomarker for thrombosis and clinical outcome in MPN.Methods:cfDNA was measured by fluorometry using PicoGreen.Results:Circulating cfDNA was elevated in MPN (n=107) vs. controls (P