Effect of Resveratrol on renal epithelial cell adaptation to hipertonicity

RUBINSZTAIN, MARÍA NATALIA; ARTUCH, AYELEN; ERJAVEC, LUCIANA; FERNÁNDEZ, MARÍA DEL CARMEN; CASALI, CECILIA; LEPERA, LEANDRO

Paraná

Congreso; LIV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; 2018

Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular, CONICET

Resveratrol(trans-3,4′,5-trihydroxystilbene, Rsv) is a small polyphenolmolecule present in a large variety of plants such as mulberries,peanuts and grapes. Rsv was widely used by Asian medicine for fungal,inflammation, hypertension, allergy, cancer and lipid diseases. In occident, it is widely used by population as dietary supplement dueto its antioxidant property. Among the molecular effects of Rsv, the activation of AMPK and SIRT1 have been reported. Rsv-induced SIRT1 activation is responsible for NF-B transcriptional activity down regulation and COX-2 expression decrease. It was also reported that Rsv has renoprotective actions.These observations are contradictory to our previous results showing that COX-2 expression is a key factor for renal cell survival and adaptation to changes in environmental osmolality. In the present work, we evaluated whether Rsv affects the adaptation of renal cells to hyperosmolarity. MDCK cells were subjected to high-NaCl media (512mOsm/kg 24h) in the absence or presence of different concentrations of Rsv (12.5,25, 50, 100 µM). After treatment, cells were collected, counted and viability determined. COX-2, and other osmoprotective genes (BGT1,SMIT and AR) expression were determined. Rsv decreased the number of cells recovered after treatment in a concentration-dependent way. Rsv also affected cell morphology hindering the formation of the typical monolayer. We also found that Rsv decreased COX-2 expression and modulated osmoprotective gene. These results clearly indicate that Rsv impedes renal cells adaptation and survival to hyperosmolality by blocking key genes expression.