Immunohistochemical biomarker profile in chronic traumatic ulcers could be associated with early carcinogenesis

PANICO R; BRUNOTTO M; GILLIGAN G; PIEMONTE E; DITADA C

Roma

Congreso; 7th WORLD CONGRESS of the International Academy of Oral Oncology; 2019

International Academy of Oral Oncology

The aim of this study is to identify a biomarker pattern of differentiation (Ck19) and cell proliferation (Ki67) in oral CTU(chronic traumatic ulcer)to those observed in Oral Squamous Cell Carcinoma (OSCC) and Potentially Malignant Disorders (PMD). Study design: a Cross-sectional study with adult patients (n = 110), both sexes, with CTU (n=41), OPMD (n=31) and OSCC (n=38) were included. CK19 and Ki67 Immunohistochemistry were carried out in specimens of the lesions and the patterns and scores of immunostaining were described according to previous publications in Negative, Low (10% or less of positive cells), Medium (10-30% of positive cells) and High (more than 30% of positive cells). The present study was approved by the Ethics Committee of the College of Dentistry, Universidad Nacional de Cordoba. Results:As it was expected, OSCC showed anoverexpression of both markers, while a CK19 focaland suprabasal pattern was found in CTU, with a lower expression of Ck19 in the OPMD group. Ki67 was found with strong intensity in basal and parabasalepithelial cells of OPMD, while only 15% of CTU showed high scores of cell proliferation (Ki67). Regarding clinical findings, the lateral tongue border was the site with the highest scores of immunostaining in CTU and OSCC group.Conclusion: there is no scientific literature on biomarker patterns of CTU. Therefore, the described patternis novel and could be indicative of a relationship between the presence of CTU, produced by chronic mechanical irritation, and oral carcinogenesis. Further studies are needed to completely understand the relationship between CMI and OSCC as well as the underlying mechanisms that could link chronic trauma with a multifactorial field of oral carcinogenesis.