GAMMA CARBONIC ANHIDRASE LIKE PROTEINS ARE PRESENT IN MITOCHONDRIAL COMPLEX I OF THE DIATOM PHAEODACTYLUM TRICORNUTUM

CAINZOS MAXIMILIANO; MARCHETTI, MARIA FERNANDA; PAGNUSSAT G; EDUARDO JULIAN ZABALETA

Salta

Congreso; LV reunión anual SAIB-PABMB; 2019

SAIB

Diatoms are a major group of algae responsable for 20% of CO2 global fixation. Phaeodactylum tricornutum is commoly used in molecular biology as a nodel organisms for understanding the biology of diatoms. Diatoms are the result of a symbiosis between an ancestral heterotrophic organism (called the exosymbiont) with a red alga (known as the endosymbiont) from which has only the chloroplast left. Diatom mitochondria are then believed to derive from the exosymbiont, i. e. the "animal" part. However, these organisms seem to contain an ancestral OXPHOS (Mitochondrial Oxidative Phosphorylation System) different form that of Opisthokonta (Animals and Fungi). In support of this, genes encoding gamma type carbonic anhydrases that were shown to be intrinsic complex I subunits n plants and amoeba but not in animals and fungi were found in all eukaryotes lineages. Here, we show that the diatom complex I is a large complex containing gamma type carbonic anhydrase subunits, supporting an ancestral origin.