Expression of recombinant fatty acid desaturase in a bovine mammary gland cell line induces changes in lipid profiles.

FILI, AE; OPIZZO, BA; HEREDIA, RM; FORCATO, DO; LUCHESSI, G.I; KUES, W; BOSCH, P

Congreso; Reunión anual de sociedades biocientíficasS A I C, S A F E, S A B, SAP; 2019

Vertebrates are unable to synthesize a subset of polyunsaturated fatty acids (PUFA) known as omega 3 and omega 6lipids. The nematode C. elegans is able to synthesize them thanks to a family of lipid desaturases (delta desaturases,i.e., FAT2 desaturase). Our hypothesis proposes that heterologous expression of a delta 12 desaturase in a bovinemammary gland cell line (MAC-T) will induce synthesis of PUFA.The aim of the present work was to transpose the C.elegans fat2 gene into the genome MAC-T and to study the resulting PUFA profile.