Cysteinyl leukotriene C4 trigger an ?immature? state on DC able to induce a inflammatory response

ALVAREZ CAROLINA; AMARAL MARÍA M; SALAMONE, GABRIELA; NAHMOD, KAREN; GEFFNER, JORGE; VERMEULEN, MÓNICA

Buenos Aires

Congreso; First French - Argentine Immunology Congress LVIII Reunión Anual de la Sociedad Argentina de Inmunología. XIII Jornada Científica del Grupo Rioplatense de Citometría de Flujo. 3º Jornadas Argentinas de Inmunodeficiencias Primarias (SAP); 2010

SAI-French Society of Immunology

Previously, we proved that cysteinil leukotriene C4 (CysLT) increases dendritic cell (DC) uptake and inhibited IL-12p70 production by mature DC. Here, we focused on the cytokine profile induced by CysLT on immature and mature DC and the mechanism involved in this modulation. For this, DC were obtained from C57BL/6 bone marrow progenitors in the presence of GM-CSF. Then, they were incubated without or with LPS (1µg/ml) for 30 min at 37ºC and treated or untreated with LTC4 (0.01 μM) overnight at 37ºC. Finally, we analyzed in culture supernatants the presence of IL-10, IL- 12p40 by ELISA. We showed that CysLT inhibited the production of IL-10 (IL-10: pg/ml mean±SEM; Ct 56±5.7; CysLT: 63±7.1; LPS: 89±7.3; LPS+CysLT: 60±5.2, *p≤0.05. n=6), whereas it stimulated the IL-12p40 secretion by mature DC (IL-12p40: pg/ml mean± SEM; Ct:43.33 ±13.17; CysLT: 32.06±9.4; LPS:93.51±23.31; LPS+CysLT,139.5±22.13, N=8; ***p