IMMUNOHISTOCHEMICAL DETECTION OF PROLACTIN AND PROLACTIN RECEPTOR IN CANINE AND FELINE PLACENTAE.

HERNÁNDEZ R, BARBEITO CG, DIESSLER

Congreso; International Federation of Placenta Associations Meeting, Buenos Aires, Argentina September 10 - 13, 2019; 2019

SLIMP-IFPA

Objectives: The aim was to determine the distribution of prolactin and itsreceptor in the endotheliochorial placentae of dogs and cats.Methods: Samples from different placentae from three dogs and two catswere processed by histological routine techniques. For immunohistochemistry,sections were deparaffinized and rehydrated; endogenousperoxidase was inhibited and antigen retrieval was carried out. For prolactindetection, a rabbit polyclonal anti-prolactin antibody was used (1/100, Abcam®, AB186522), whereas to detect prolactin receptor (PLR) amouse monoclonal antibody was used (1/100, Abcam®, AB2772). Bothantibodies were incubated overnight at 4ºC. Slides were incubated withanti-rabbit or anti-mouse EnVision? detection system, respectively; 3,3-diaminobenzidine tetrahydrochloride was used as chromogen.Results: In dog placenta, prolactin was mainly evidenced in maternalendothelium and connective tissue (from endo, myo and perimetrium),while PLR was noticed in uterine gland epithelium (both deep and superficial),smooth myocytes (myometrium and vessels) and maternalendothelium. In cat placenta, decidual cells strongly expressed both prolactinand PLR, even those deep, small, early differentiated decidual cells.Glandular epithelium was unevenly labelled in this species.