Decidualized cells respond differentially contributing to a tolerogenic microenvironment according to blastocyst quality

FERNANDEZ, LAURA; SOCZEWSKI, ELIZABETH; GORI, SOLEDAD; SABBIONE, FLORENCIA; PAPARINI, DANIEL E.; VOTA, DAIANA M.; MARTINEZ, GUSTAVO; PÉREZ LEIRÓS, CLAUDIA; GRASSO, ESTEBAN N.; RAMHORST, ROSANNA

Mar del Plata

Congreso; SAIC-SAI-SAFIS; 2018

SAIC-SAI-SAFIS

During decidualization process endometrial stromal cells modulate their secretome and display the ability to secrete many immuneregulatory and implantatory factors. Based on this ability, decidualized cells sense embryo quality allowing the implantation of normal development (ND) blastocysts while limit those with impaired development (ID). We propose that decidualized cells promote a tolerogenic microenviroment in response to ND blastocyst-derived factors; in contrast, decidualized cells response to ID-blastocyst derived factors sustaining a hostile microenvironmet that could trigger menstruation. Here we used an in vitro decidualization model based on the Human endometrial stromal cell line (HESC) treated with medroxiprogesterone+dbcAMP in absence/presence of Blastocyst Conditioned Media (BCM) for 24h. BCM were recovered from 5 days individually cultured blastocyst obtained from women with IVF or ICSI indication according to male evaluation, and classified as ND or ID blastocyst according to clinical standards. We observed that ND-BCM increased the frequency of decidualized cells producing the inmunomodulator peptide VIP evaluated by FACS, while ID BCM decreased expression of its receptor VPAC1 relative to decidualized cells not treated with BCM (p