Response to extracellular ATP is dependent on the expression of AQP2 in renal cells.

ALEJANDRO PIZZONI; DI GIUSTO, GISELA; LOPEZ GONZALEZ MACARENA; VALERIA RIVAROLA; CAPURRO CLAUDIA; P. FORD

Congreso; SAFIS-SAIC 2018; 2018

Extracellular ATP is a physiological signal that can activate P2 re- ceptors and trigger intracellular Ca2+ behavior, including migration. We have previously described in renal cells that intracellular Ca2+ signals can be modulated by the 5394. 3UHYLRXV ZRUNV IURP RXU ODERUDWRU KDYH DOVR VKRZQ WKDW $432 H[SUHVVLRQ DFFHOHUDWHV FHOO PRWLOLW DQG SUROLIHUDWLRQ. 7KH REMHFWLYH RI WKH SUHV- HQW ZRUN ZDV WR VWXG WKH LQÁXHQFH RI $432-75394 LQWHUDFWLRQin Ca2+ signals elicited by extracellular ATP and its consequencesLQ FHOO PLJUDWLRQ. :H XVHG WZR UDW FRUWLFDO FROOHFWLQJ GXFW FHOO OLQHVexpressing AQP2 (AQP2-RCCD1) or not (WT-RCCD1). We studied Ca2+ signals using FURA-2 in response to 20 µM of extracellular ATP. We found that Ca2+ signals in WT-RCCD cells are greater than in AQP2-RCCD1 FHOOV ($UHD 8QGHU WKH &XUYH; $8&WT: 401 ? 17 vs. AUCAQP2: 272 ? 10; Q 318 FHOOV RI 7 LQGHSHQGHQW H[SHULPHQWV.pSULGR[DOSKRVSKDWH-6-D]RSKHQO-2¿,4¿-GLVXOIRQLF DFLG@ 100 ?0). +&-067047 (1 ?0), D VSHFLÀF 75394 LQKLELWRU, RQOdecreases the response of WT-RCCD1 cells. We also studied cellPLJUDWLRQ LQ WKH SUHVHQFH RI H[WUDFHOOXODU $73 XVLQJ WKH ZRXQG KHDO-ing assay. We found that ATP stimuli augments migration only inFHOOV WKDW GR QRW H[SUHVV $432 (% RI ZRXQG FORVXUH DUHD; :7CTRL:34.9 ? 1.2 % YV. :7ATP: 47.6 ? 3.8 %; 3 LQGHSHQGHQW H[SHULPHQWV. S