Cells derived from renal proximal epithelia are affected by extracellular pH in a different way than cells derived from clear renal cell carcinoma: Effect of NHE1.

MARINA MAZZOCCHI; G. DI GIUSTO; MICAELA PORTA; ALEJANDRO PIZZONI; BELTRAMONE, NATALIA; FORD P; CAPURRO CLAUDIA

Congreso; SAFIS-SAIC 2018; 2018

The association between cell death and intracellular pH elicits thepossibility that extracellular pH (pHe) may modify cell survival. Moreover,as tumor extracellular acidity is a hallmark of cancers, is probablethat pHe affects differently cancer or normal cells. The aim of thisstudy was to investigate whether exposure to acidic or alkaline mediaaltered cell survival in two renal cell models: HK2, derived fromnormal human proximal epithelial cells and 786-O, derived from humanrenal clear cell carcinoma. Cells were exposed for 24h to mediawith different pHe and then cell survival was estimated by acrydinorange?ethidium bromide experiments. Our results show that HK2cell survival decays with an acidiication of 0.1 pH units (Δ Cell deathat pH 7.3 = 14.30 ± 3.00, p< 0.05 n=5) while 786-O cells can surviveeven at pH 6.4 (Δ Cell death at pH 6.4 = 0.24 ± 0,50 ns n=5). Onthe other hand 786-O cells were more sensible to alkalinization than HK2 cells. While an alkalinization of 0.1 affected only malignant cells(Δ Cell death at pH 7.5 HK2= 0.03 ± 0.50 vs 786-O= 10.57 ± 2.00 p