Synthesis, characterization and radiosensitizing effect of magnetic nanoparticles in melanoma cells.

C. GRISSI; M. TAVERNA PORRO; M. ATIA; L. NEGRIN; M.F. DEL GROSSO; H. DURÁN; I. IBAÑEZ

Congreso; Reunión Conjunta de Sociedades de Biociencias. , LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2017

Malignant melanoma (MM) is the most deadly skin cancer, is highlymetastatic and resistant to conventional therapies. Advances innanomaterials contributed in recent years to develop new strategiesfor cancer treatment. In this context, magnetic nanoparticles (MNPs)exhibit great potential for diagnosis and therapy. MNPs are highlybiocompatible, stable and can be directed under an external magneticfield. Moreover, incubation of cells with MNPs can increase intracellularlevels of reactive oxygen species (ROS). Based on the highendogenous ROS levels of melanoma cells, we propose that combinedtreatment with MNPs and ionizing radiation (IR) could inducea cytotoxic oxidative stress by surpassing antioxidant capacity andsensitizing radioresistant melanoma cells. To test this hypothesis,the aim of this study was to synthesize, characterize and evaluatethe radiosensitizing effect of MNPs in A375 human melanoma cells.Iron oxide MNPs were synthesized by the coprecipitation of Fe2+and Fe3+ method and stabilized by methyl-poly(ethylene glycol).MNPs characterization by transmision electron microscopy, X-raydiffraction, magnetometry and Mössbauer spectroscopy showedsuperparamagnetic behavior and low dispersion in shape and sizes(8-17 nm). No cytotoxicity was observed by MTT assay in A375cells exposed up to 250 μg/ml for 24 h. DCFH assay revealed higherROS levels in A375 cells after MNPs treatment (p