INVESTIGADORES
SAMPIETRO Diego Alejandro
congresos y reuniones científicas
Título:
ANTIFUNGAL ACTIVITY OF A METABOLITE PURIFED FROM Terminalia triflora
Autor/es:
CORONEL, R.S.; ANGOS, A.; SGARIGLIA, M.A.; SAMPIETRO, D.A.; SOBERON, J.R.
Lugar:
TAFI DEL VALLE
Reunión:
Congreso; XXXV JORNADAS DE LA ABT; 2018
Institución organizadora:
ASOCIACION DE BIOLOGIA DE TUCUMAN
Resumen:
Current human fungal infection therapies due to Candida albicans are limited by fungistatic action, toxicity or development of resistance. Terminalia triflora (Tt), a species used as an antifungal in tradicional medicine, is a potential source of natural antifungal compounds. Tt leaves were extracted with ethanol, and successively fractionated with solvents with increased polarity, chromatography, solid phase extraction and HPLC to yield an antifungal compound (P1). P1 antifungal activity against C. albicans (ATCC10231) was tested. Minimal inhibitory concentration (MIC) and fractional inhibitory concentration index (FICI) were obtained through microdilution and cell viability assay for P1 alone and in combination with fluconazole (FLU). P1 (100µg/mL) was active against C.albicans only in combination with FLU (0.5µg/mL). FICI=0.258 indicated synergistic effect between P1 and FLU. P1 + FLU resulted in a fungicidal combination. These results support the ethnomedicinal applications described forTt.
