GABAB receptor deletion in kiss1 neurons/cells affects the hypothalamic expression of kiss1 in male mice

SURKIN, PABLO N.; BIZZOZZERO HIRIART, MARIANNE; REPETTO, M; BETTLER B; LIBERTUN C; LUX LANTOS V; DI GIORGIO N

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias; 2020

SAIC

It is known that Kiss1 cells/neurons co-express GABAB receptors (GABABRs), and our group has previously demonstrated that global GABAB1KO mice show a dramatic increase in Kiss1 expression in male and female extrahypothalamic areas (amygdala, BNST, lateral septum), but its expression does not differ in AVPV-PeN/ARC.To establish the impact of GABABRs on kisspeptin physiology we developed a strain of mice with specific deletion of GABABRs in Kiss1 cells/neurons and characterized them from reproductive perspectives.Kiss-Cre mice (Jackson?s Lab) were crossed with GABAB-floxed mice (donated by Dr. Bettler) to obtain Kiss1-GABAB1KO (KO) mice. Male Kiss1 expression was evaluated by qPCR in amygdala, BNST, AVPV-PeN and ARC micropunches; serum hormones were determined by RIA.Conversely to GABAB1KO mice, no significant changes were observed in extrahypothalamic areas [amygdala (fold change): KO=0.95±0.08 (n=10) vs WT=0.98±0.08 (n=9), ns; BNST (fold change): KO=3.36±1.48 (n=9) vs WT=1.05±0.15 (n=6), p=0.07]. Interestingly, KO males showed increased Kiss1 expression in the ARC [fold change: KO=1.32±0.16 (n=8) vs WT=0.9±0.1 (n=8), p