FIRST REPORT OF GENOME SEQUENCING, ASSEMBLY AND GENE PREDICTION OF TRICHODERMA KONINGIOPSIS

CASTRILLO ML; BICH GA; FERNÁNDEZ T; MEGRIAN D; AMERIO NS; SOAREZ JN; SAPARRAT MCN; ZAPATA PD; NAYA HM; VILLALBA LL

Posadas

Congreso; VIII Argentinian Bioinformatics and Computational Biology Congress; 2017

The genus Trichoderma Pers. (Ascomycota, Sordariomycetes, Hypocreales) includes several saprotrophic forms. They are capable of growing on different organic substrates such as wood and bark as well as ones from other fungi. This is due to its high versatility to colonize and attack under a broad spectrum of ecological conditions. They may be beneficial to industry, agriculture and medicine as well as other fields. Therefore, these fungi are important biotechnologically tools. However, there are several species of this genus remain to be characterized at physiological, biochemical, and genetical level. The advent of methodologies using genomic and transcriptomic data is currently a promosing option for giving a better understanding of Trichoderma species diversity and their potential use as biotechnological tools. In this work, we report the whole-genome shotgun sequencing, assembly and gene prediction of the Trichoderma koningiopsis POS7 strain, a fungus with an outstanding enzymatic activity. T. koningiopsis POS7 strain was isolated from wood collected from a forest environment of Misiones (Argentina; 27°24?31.8??S, 55°53?48.5??W). Genomic DNA library and draft genome sequencing was performed by Macrogen Co. (Korea) using the Illumina MiSeq platform. Genomic DNA library was prepared using a rapid shotgun library. Quality control procedures removed DNA spike-in, artifacts and ambiguous or low quality reads. Paired ends having at least 90% of bases with quality score greater or equal to Q20 were filtered before assembly. The next-generation sequencing libraries resulted in 7,773,936 paired reads (36,586,254 bp) with an approximate insert size of 100 bp. These sequences were assembled in 248 scaffolds using SPAdes software. The resulting genome sequence of T. koningiopsis POS7 had an estimated size of 36.6 Mb and a GC content of 48.82%. To predict genes in the T. koningiopsis POS7 genome we used ab initio gene predictor, Augustus and FgenesH softwares. These procedures determined that 88 of the 248 scaffolds presented gene prediction and included 11,644 protein-coding genes. The fungus with an outstanding enzymatic activity, T. koningiopsis POS7 strain, has a genome of 36.6 Mbp with a GC content of 48.82%, being predicted 11,644 coding genes.