INVESTIGADORES
RODRIGUEZ TALOU Julian
congresos y reuniones científicas
Título:
Methyl jasmonate elicitation and reactive oxygen species production in Rubia tinctorum cell suspension cultures
Autor/es:
PERASSOLO, M; QUEVEDO, CV; BUSTO, VD; CARDILLO AB; MARTÍNEZ, CA; GIULIETTI, AM; RODRIGUEZ TALOU , J
Lugar:
Barcelona
Reunión:
Congreso; 14th European Congress on Biotechnology,; 2009
Institución organizadora:
European Federation of Biotechnology
Resumen:
Elicitation is a useful strategy to induce accumulation of secondary
metabolites in plants. Besides the production of secondary
metabolites, the defense response against elicitation also triggers
the generation of reactive oxygen species (ROS), and can
also lead to cell death. In particular, methyl jasmonate (MeJ)
is a well-described agent that has been widely used to enhance
anthraquinone (AQs) production in Rubia tinctorum. AQs biosynthesis
involves different metabolic routes: o-succinylbenzoate,
precursor of A and B rings, is derived from -ketoglutarate and
isochorismic acid. This is produced by the isochorismate synthase
from chorismic acid, which is the end-product of shikimate pathway.
C ring is derived from the terpenoid pathway. It has been
proposed that proline cycle could be coupled to the pentose phosphate
pathway (PPP) as the NADP+ generated by proline reduction
from glutamate could act as cofactor of the first enzymes of the
PPP. This pathway generates erithrose-4-phosphate, the substrate
of the shikimate pathway. The aim of the present work was to
study the relationship between MeJ elicitation, ROS, proline cycle
and anthraquinone production in R. tinctorum cell suspension cultures.
Cell cultures were treated with MeJ, diphenyl iodonium
(DPI, an inhibitor of H2O2 generation) and a glucose/glucose oxidase
system (Glu/GOD, a H2O2 generating-system). After 48 hours
of culture, MeJ increased AQs content (53%, P < 0.05) compared to
control cells, but not after 24 hours. Treatment with DPI of both
control and MeJ-treated cells showed less AQs accumulation at 24
(26% and 33% less) and at 48 hours of culture (18.5% and 51%
less, compared to both treatments without DPI). The Glu/GOD
system induced AQs production (14 and 48% more than the control
treatment, after 24 and 48 hours of culture). MeJ treatment
increased proline content at 48 hours of culture (22% more), while
it was decreased when DPI was added to MeJ-treated cells (28%
less). Relationship between ROS and elicitation is currently under
study.Rubia tinctorum. AQs biosynthesis
involves different metabolic routes: o-succinylbenzoate,
precursor of A and B rings, is derived from -ketoglutarate and
isochorismic acid. This is produced by the isochorismate synthase
from chorismic acid, which is the end-product of shikimate pathway.
C ring is derived from the terpenoid pathway. It has been
proposed that proline cycle could be coupled to the pentose phosphate
pathway (PPP) as the NADP+ generated by proline reduction
from glutamate could act as cofactor of the first enzymes of the
PPP. This pathway generates erithrose-4-phosphate, the substrate
of the shikimate pathway. The aim of the present work was to
study the relationship between MeJ elicitation, ROS, proline cycle
and anthraquinone production in R. tinctorum cell suspension cultures.
Cell cultures were treated with MeJ, diphenyl iodonium
(DPI, an inhibitor of H2O2 generation) and a glucose/glucose oxidase
system (Glu/GOD, a H2O2 generating-system). After 48 hours
of culture, MeJ increased AQs content (53%, P < 0.05) compared to
control cells, but not after 24 hours. Treatment with DPI of both
control and MeJ-treated cells showed less AQs accumulation at 24
(26% and 33% less) and at 48 hours of culture (18.5% and 51%
less, compared to both treatments without DPI). The Glu/GOD
system induced AQs production (14 and 48% more than the control
treatment, after 24 and 48 hours of culture). MeJ treatment
increased proline content at 48 hours of culture (22% more), while
it was decreased when DPI was added to MeJ-treated cells (28%
less). Relationship between ROS and elicitation is currently under
study.o-succinylbenzoate,
precursor of A and B rings, is derived from -ketoglutarate and
isochorismic acid. This is produced by the isochorismate synthase
from chorismic acid, which is the end-product of shikimate pathway.
C ring is derived from the terpenoid pathway. It has been
proposed that proline cycle could be coupled to the pentose phosphate
pathway (PPP) as the NADP+ generated by proline reduction
from glutamate could act as cofactor of the first enzymes of the
PPP. This pathway generates erithrose-4-phosphate, the substrate
of the shikimate pathway. The aim of the present work was to
study the relationship between MeJ elicitation, ROS, proline cycle
and anthraquinone production in R. tinctorum cell suspension cultures.
Cell cultures were treated with MeJ, diphenyl iodonium
(DPI, an inhibitor of H2O2 generation) and a glucose/glucose oxidase
system (Glu/GOD, a H2O2 generating-system). After 48 hours
of culture, MeJ increased AQs content (53%, P < 0.05) compared to
control cells, but not after 24 hours. Treatment with DPI of both
control and MeJ-treated cells showed less AQs accumulation at 24
(26% and 33% less) and at 48 hours of culture (18.5% and 51%
less, compared to both treatments without DPI). The Glu/GOD
system induced AQs production (14 and 48% more than the control
treatment, after 24 and 48 hours of culture). MeJ treatment
increased proline content at 48 hours of culture (22% more), while
it was decreased when DPI was added to MeJ-treated cells (28%
less). Relationship between ROS and elicitation is currently under
study.+ generated by proline reduction
from glutamate could act as cofactor of the first enzymes of the
PPP. This pathway generates erithrose-4-phosphate, the substrate
of the shikimate pathway. The aim of the present work was to
study the relationship between MeJ elicitation, ROS, proline cycle
and anthraquinone production in R. tinctorum cell suspension cultures.
Cell cultures were treated with MeJ, diphenyl iodonium
(DPI, an inhibitor of H2O2 generation) and a glucose/glucose oxidase
system (Glu/GOD, a H2O2 generating-system). After 48 hours
of culture, MeJ increased AQs content (53%, P < 0.05) compared to
control cells, but not after 24 hours. Treatment with DPI of both
control and MeJ-treated cells showed less AQs accumulation at 24
(26% and 33% less) and at 48 hours of culture (18.5% and 51%
less, compared to both treatments without DPI). The Glu/GOD
system induced AQs production (14 and 48% more than the control
treatment, after 24 and 48 hours of culture). MeJ treatment
increased proline content at 48 hours of culture (22% more), while
it was decreased when DPI was added to MeJ-treated cells (28%
less). Relationship between ROS and elicitation is currently under
study.R. tinctorum cell suspension cultures.
Cell cultures were treated with MeJ, diphenyl iodonium
(DPI, an inhibitor of H2O2 generation) and a glucose/glucose oxidase
system (Glu/GOD, a H2O2 generating-system). After 48 hours
of culture, MeJ increased AQs content (53%, P < 0.05) compared to
control cells, but not after 24 hours. Treatment with DPI of both
control and MeJ-treated cells showed less AQs accumulation at 24
(26% and 33% less) and at 48 hours of culture (18.5% and 51%
less, compared to both treatments without DPI). The Glu/GOD
system induced AQs production (14 and 48% more than the control
treatment, after 24 and 48 hours of culture). MeJ treatment
increased proline content at 48 hours of culture (22% more), while
it was decreased when DPI was added to MeJ-treated cells (28%
less). Relationship between ROS and elicitation is currently under
study.2O2 generation) and a glucose/glucose oxidase
system (Glu/GOD, a H2O2 generating-system). After 48 hours
of culture, MeJ increased AQs content (53%, P < 0.05) compared to
control cells, but not after 24 hours. Treatment with DPI of both
control and MeJ-treated cells showed less AQs accumulation at 24
(26% and 33% less) and at 48 hours of culture (18.5% and 51%
less, compared to both treatments without DPI). The Glu/GOD
system induced AQs production (14 and 48% more than the control
treatment, after 24 and 48 hours of culture). MeJ treatment
increased proline content at 48 hours of culture (22% more), while
it was decreased when DPI was added to MeJ-treated cells (28%
less). Relationship between ROS and elicitation is currently under
study.2O2 generating-system). After 48 hours
of culture, MeJ increased AQs content (53%, P < 0.05) compared to
control cells, but not after 24 hours. Treatment with DPI of both
control and MeJ-treated cells showed less AQs accumulation at 24
(26% and 33% less) and at 48 hours of culture (18.5% and 51%
less, compared to both treatments without DPI). The Glu/GOD
system induced AQs production (14 and 48% more than the control
treatment, after 24 and 48 hours of culture). MeJ treatment
increased proline content at 48 hours of culture (22% more), while
it was decreased when DPI was added to MeJ-treated cells (28%
less). Relationship between ROS and elicitation is currently under
study.P < 0.05) compared to
control cells, but not after 24 hours. Treatment with DPI of both
control and MeJ-treated cells showed less AQs accumulation at 24
(26% and 33% less) and at 48 hours of culture (18.5% and 51%
less, compared to both treatments without DPI). The Glu/GOD
system induced AQs production (14 and 48% more than the control
treatment, after 24 and 48 hours of culture). MeJ treatment
increased proline content at 48 hours of culture (22% more), while
it was decreased when DPI was added to MeJ-treated cells (28%
less). Relationship between ROS and elicitation is currently under
study.