INVESTIGADORES
CALZADILLA Pablo Ignacio
congresos y reuniones científicas
Título:
CHLOROPLASTIC ACCLIMATION UNDER COLD STRESS IN LOTUS JAPONICUS
Autor/es:
CALZADILLA P.I; VILAS, JUAN MANUEL; UNREIN, FERNANDO; MAIALE S.J.; RUIZ O.A.
Lugar:
Corrientes
Reunión:
Congreso; XXXI Reunion Argentina de Fisiologia Vegetal; 2016
Institución organizadora:
Sociedad Argentina de Fisiologia Vegetal
Resumen:
Photosynthesis is considered an important source of ROS in plants, and disruption of PSII activity may increase its production. This phenomena leads to photoinhibition, a process accelerated by low temperatures. With the aim to address the photosynthetic acclimation response of L. japonicus under cold stress, two ecotypes (MG-1 and MG-20) were studied. Previous data showed that photoinhibition occurs in stress and differentially between ecotypes, being MG-1 more affected than MG-20. In this study, we used flow cytometry and confocal microscopy to evaluate chloroplast ROS generation under control and stress conditions. Our results showed that chloroplast ROS generation was higher in the low temperature treatment compared to control, only for MG-1, and after 7 d of stress. Chloroplast ultrastructure was also evaluated by Transmission Electron Microscopy. An increased in starch granules and chloroplast swelling was observed due to chilling, and differentially between ecotypes. These effects were further enhanced with the increased time of cold exposure. Thylakoid membrane distortion and plastoglobule presence were also observed due to the stress condition.As a conclusion, data showed that the differential photosynthetic acclimation of both L. japonicus ecotypes to cold stress could be, at least partially, explained by their chloroplastic redox imbalance and ultrastructure changes.