Pituitary TGFβ1 system during postnatal development

ABELEDO, ALEJANDRA; CAMILLETTI, MARIA ANDREA; FARAONI, ERIKA; PISERA, DANIEL; FERRARIS, JIMENA; DÍAZ-TORGA, GRACIELA

Orlando

Congreso; ENDO 2017; 2017

Endocrine Society

TGFβ1 is a known inhibitor of lactotroph cell proliferation and Prolactin (PRL) secretion. TGFβ1 bioavailability is tightly regulated by different components of the ?TGFβ1 system? including latent binding proteins (LTBPs), local activators, and TGFβ receptors. Pituitary TGFβ1 activity is regulated by dopamine (DA) and estradiol (E2). We have previously demonstrated that DA stimulates and E2 inhibits the expression of most of the component of the pituitary TGF1 system (1). The aim of the present work was to study the ontogeny of the pituitary TGF1 system in male and female Sprague Dawley rats at different postnatal ages: 11, 23 and 45 days. Adult females were used in diestrus. ELISAs were performed to quantify total and active TGFβ1 content in pituitary homogenates. We evaluated the expression of the components of TGFβ1 system by qPCRs. Serum prolactin was assayed by RIA. The mRNA expression of most of the components of the TGFβ1 system were found significantly increased in pituitaries from 11d pups: TβRII, ALK1, SMAD7, SMAD4 and the latent proteins LTBP1 and LTBP3, compared to adults, without gender differences (p