Improvement of thiol redox homeostasis in glaucomatous primary visual cortex after lipoic acid administration

REIDES, CLAUDIA; HVOZDA ARANA AILEN; LASAGNI VITAR, ROMINA; FERREIRA, SANDRA; LLESUY, SUSANA

Mar del Plata

Congreso; Reunión de la Sociedad Argentina de Investigaciones Clínicas (SAIC); 2018

Lipoic acid has been used to ameliorate oxidative stress in glaucoma as it scavenges reactive oxygen and nitrogen species, repair oxidized proteins and lipids, and regenerate endogenous antioxidants.The aim of this work was to evaluate if thiol redox homeostasis improved after lipoic acid administration in visual cortex in a glaucoma rat model. Wistar rats (3 months) were divided in four groups (n=24): glaucoma in which rats were operated under a microscope by cauterized two of the episcleral veins (G), glaucoma treated with lipoic acid 100 mg/kg i.p. (LG), control which received a sham procedure (C) and control treated with lipoic acid 100 mg/kg i.p. (LC). Seven days after surgery, rats were euthanized, brains were removed and visual cortex was separated. The following markers were evaluated: reduced glutathione (GSH), oxidized glutathione (GSSG), redox index (GSH/GSSG), thioredoxin reductase (TrxR), glutathione reductase (GR), glutathione peroxidase (GPx), and glucose-6-phosphate deshidrogenase (G6PD). Comparing G to C: GSH decreased 46 % (p< 0.001), GSSG increased 26 % (p< 0.05), redox index diminished 57 % (p< 0.001), TrxR decreased 34 % (p