Preparation, characterization and wound healing effects of acyclovir-loaded chitosan/hyaluronic acid polymeric films for skin Herpes

VALENZUELA-OSES, JOHANNA; JIMENEZ KAIRUZ, ALVARO; GARCÍA, MÓNICA C

Lima

Congreso; Simposio Internacional en Biotecnología Microbiana y Salud; 2019

Grupo de Investigación en Biotecnología Bacteriana y Salud, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos

INTRODUCTIONHerpes is a disease caused by a type of virus belonging to the family Herpes viridae that represents an important public health problem worldwide INTRODUCTION Polymeric films based on drug-interpolyelectrolyte complexes (DIPEC) represent an interesting therapeutic strategy that allows controlled drug release, providing a prolonged effect 2. The aim of this work was to develop and characterize acyclovir-loaded polymeric films for topical treatment of skin wounds by herpes. MATERIALS AND METHODS DIPEC dispersions were prepared by coacervation method. First, chitosan (Ch) was partially neutralized with HCl 1N, followed the addition of acyclovir (ACI) to neutralize 50% of ionizable amine groups of Ch. Then, sodium hyaluronate (HA) 0.005 M was added to neutralize 10% of ionizable amine groups of Ch under constant stirring at 14.000 rpm for 15 minutes. DIPEC dispersions were characterized by DLS, seta potential (ZP) hydrodynamic diameter (Dh), polydispersity index, pH measurements and ionic pattern of an amorphous solid. Moreover, image of optical microscopy showed birefringence phenomenon for PM and ACI, while the DIPEC could not be observed under polarized light due to their amorphous nature. The films obtained had excellent ACI content uniformity with < 5% of coefficient of variation. A higher sorption capacity was observed in both unloaded and loaded films. In presence of the films the zeta potential of mucin switched from negative to positive, which indicated that electrostatic interactions occur between the films and mucin, thus revealing their bioadhesive properties. In vit ro release studies showed a controlled release of ACI from the films. CONCLUSIONPolymeric films showed promising properties for the treatment of skin wound produced by herpes virus.