Uterine vascularization in Lagostomus Maximus: Implications for embryonic survival

FRANCISCO ACUÑA , CLAUDIO GUSTAVO BARBEITO , ENRIQUE LEO PORTIANSKY , MARÍA ANGÉLICA MIGLINO , MIRTA ALICIA FLAMINI

CABA

Simposio; LASDB2019 Simposio Latinoamericano de Biología de Desarrollo 2019; 2019

Latinoamerican Society Development Biology

Pregnancy requires adequate uterine vascularization at implantation and postimplantation time to facilitate th e transport of nutrients and oxygen to the embryo.In mammals, it was observed that the absence of uterine vascular flow generatesembryonic death. In Lagostomus maximus, 10 12 blastocysts are implanted,although only the 2 implanted near the cervix will b e viable; the rest dies fromunknown causes. Our goal was to determine whether a differential vascularizationin the endometrium and myometrium along the uterine horns (UH) of non pregnantvizcachas could participate in the aforementioned death. Three UH w eretransversely sectioned in 7 sectors and included in paraffin. Immunohistochemistryagainst CD31 (vascular endothelium marker) was done. Digital images wereanalyzed to determine the ratio between the area occupied by blood vessels perendometrium and m y ometrium. Mean data from 3 females and for each UH sectorwas compared using ANOVA and Duncan´s test. Differences were consideredsignificant for p≤0.05. The area ratio occupied by blood vessels in both zonesincreased significantly in the craniocaudal sen se of the organ. These resultsindicate that the vascular supply received by each implantation would bedifferential, so that the cranial and medial implantations would be less vascularizedthan the caudal ones (close to the cervix). These results support the hypothesisthat vascular supply could be one of the causes of the early embryonic death ofthe species.