Proliferation of trophoblast in human term placentae cultured under high concentrations of glucose (diabetes in vitro model) and in placentae from diabetic women, infected with Trypanosoma cruzi (T. cruzi)

DÍAZ-LUJÁN CINTIA M; BENIZIO EVANGELINA L; MEZZANO LUCIANA; MOREIRA- ESPINOZA MARÍA JOSÉ; EDELSTEIN DANIELA A; FRETES RICARDO E; SALERA PABLO B; PIEGARI MARIANA; TRIQUELL MF

Otro; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA S A I C . S A F E . S A B . SAP 2 0 1 9; 2019

Placental trophoblast maintenance involves a highly regulated cellular turnover dependent on a delicate balance between cells proliferation, fusion, differentiation and death. Structural alterations of the chorionic villi caused by T cruzi have been described in vitro, but little is known about trophoblast turnover in diabetic placentae infected. Study aim: Analyze if T. cruzi infection induces trophoblast proliferation changes in human placentae cultured under normal and high concentrations of glucose and in diabetic placentae. Methods: Paraffin-embedded blocks of diabetic and normal placentae (from archive) were cultured under normal and hyperglycemic conditions with 5.5 or 25mmol/l D-Glucose, respectively (diabetes in vitro model)(COEIS 32/2016). Term human placentae were infected in vitro with 1x106 trypomastigotes (Tulahuen strain) for 24 h. Immunohistochemistry to ki67 and Cytokeratin 7 (CTK7) were performed to evaluate trophoblast proliferation and integrity, and syncytiotrophoblast (STB) detachment, perivillous fibrin deposits and syncytial knots quantity were analyzed in Hematoxiline/Eosin stained sections. Fiji ImageJ was used for image quantification and ANOVA, Tukey?s multiple comparison, and Student's t-test (p