Use of Lab on a Chip (LOC) microdevices for studying cancer stem cells. The chemotherapy response in an invasive bladder cancer model

IMANOL AGÜERO; DENISE BELGOROSKY; YANINA LANGLE; ISRAEL GARCÍA-SILVA; BETIANA LERNER; MAXIMILIANO PÉREZ; ANA MARÍA EIJÁN

Mar del Plata

Congreso; Sociedad Argentina de Investigación Clínica 2019; 2019

Bladder cancer (BCa) is one of the most common tumors of the male urogenital tract and an important cause of death. The treatment of the invasive tumor is radical cystectomy. Currently, to improve patient?s standard of living, conservative surgery followed by chemo (CT) and radiotherapy is proposed. Cancer Stem Cells (CSC) are a minority cell population associated with treatment resistance and tumor recurrence, so their quantification and identification are of special interest. Lab on a Chip (LOC) systems, have surged, in the last decade, as a powerful tool for cell individual study, with the benefit of using a low amount of biological samples. Objective: Quantify and evaluate pluripotency and tumorigenic capacity of CSC in MB49-I, an invasive BCa cell line, post-treatment with CT agents, in macro culture conditions and in LOC systems. Results: Doxorubicin (Doxo) or cisplatin (CisPt) treatment decreased the number of CSCs, measured as sphere formation efficiency (p