INVOLVEMENT OF NITRIC OXIDE AND TNF-ALPHA IN CELL DEATH AND PROLIFERATION OF GC-1 SPERMATOGONIA CELL LINE

MARÍA EUGENIA FERREIRO; CINTHIA SOLEDAD MENDEZ; JIMENA FERRARIS; CANDELA GONZALEZ; CRISTIAN MARCELO SOBARZO; PATRICIA VERÓNICA JACOBO; LIVIA LUSTIG ; MARÍA SUSANA THEAS

Mar del Plata

Congreso; REUNIÓN ANUAL 2016 SAIC-SAI-SAFE; 2016

SAIC-SAI-SAFE

Nitric oxide (NO) and Tumor Necrosis Factor alpha (TNF-α)are pleiotropic factors that display multiple biological functions. Inexperimental autoimmune orchitis (EAO), a rat model of chronicinflammation associated to infertility, spermatocytes die by apoptosisinduced by high levels of NO and TNF-α while spermatogoniathat persist in the basal compartment of the seminiferous tubulesare arrested. We propose that these factors are also involved inspermatogonia cell cycle control in EAO. To evaluate this hypothesiswe explored the effect of NO and TNF-α/TNFR system onproliferation and death of the spermatogonia GC-1 cell line.DETANonoate(DETA, a NO donor) reduced GC-1 viability evaluated bythe colorimetric assay of MTS (mean±SEM, media: 0.499±0.013;DETA 2mM:0.344±0.024**; DETA 0.5mM:0.522±0.019; DETA0.05mM:0.580±0.033 **p