Long-term effects of neonatal exposure to enviromental relevant dose of glyphosate-based herbicide on uterine decidualization

INGARAMO P; VARAYOUD J; MILESI MM; GUERRERO SCHIMPF M; MUñOZ-DE-TORO M; LUQUE EH

Mar del Plata

Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2016

Sociedad Argentina de Investigación Clínica (SAIC)

In Argentina, glyphosate-based herbicides (GBH) are commonly used to control weeds. Although regulatory agencies haveasserted that are relatively safe, reports from our laboratory suggest that GBH may have endocrine disrupting effects. The aim ofpresent work was to evaluate the long-term effects of neonatalexposure to a GBH assessing parameters related to uterine decidualization in rats. Female Wistar pups received saline solution(control, C) or an environmental relevant dose of GBH (2 mg/kg)by sc injection on postnatal day (PND) 1, 3, 5 and 7. On PND90female rats were mated with fertile non-exposed male. Pregnantrats were sacrifced on gestation day (GD) 19 and GD9 to evaluatemolecular markers in the implantation sites (IS). Desmin immunostaining was used as a marker of decidualization. The newlyformed blood vessels were detected by nestin immunostaining.The Wnt5a, Wn7a and â-catenin expressions were evaluated inIS for their key roles on decidualization. On GD19, GBH groupshowed a high incidence of fetal resorptions. On GD9, a signifcantdecrease in the decidualized area (DA) (C: 1.55±0.32 mm2 vsGBH: 0.71±0.18 mm2, p