INVESTIGADORES
RICARDI Martiniano Maria
congresos y reuniones científicas
Título:
Plant proline hydroxylation mediated by prolyl 4-hydroxylases (P4Hs) and subsequent O-glycosylation on hydroxyproline rich glycoproteins (HRGPs) is crucial for polarized growth in Arabidopsis root hairs
Autor/es:
J. M. ESTEVEZ; S. M. VELASQUEZ; M. M. RICARDI; N. D. IUSEM
Lugar:
Alexandria
Reunión:
Congreso; BioVision Alexandria 2010; 2010
Institución organizadora:
TWAS
Resumen:
From 13 plant prolyl 4-hydroxylases (P4Hs) present in Arabidopsis genome
only few of them are highly expressed in roots. Pharmacological
experimets indicated that apical cell expansion in the elongation phase
of root hairs are partially blocked by binding of a specific inhibitor
,-Dipyridil (,-DP) of prolyl 4-hydroxylases (P4Hs) to the catalytic
site of these enzymes. In addition, gene disruption of single P4Hs
highly expressed in root (p4h2, p4h5, and p4h13) resulted in drastically
reduced root hair lengths. Dual subcellular localization in the ER and
medial-Golgi suggests that hydroxylation of HRGP substrates would occur
in both organelles. This data suggests that specifically the deficiency
in hydroxylation mediated by rhP4Hs and subsequent O-glycosilation on
putative Hidroxyproline Rich Glycoproteins (HRGPs) is an important
structural factor for reduced elongation in polarized growth on root
hairs.