SHORT ALCOHOL CONSUMPTION IMPAIRS THE OFFSPRING HEALTH IN MICE

LUCILA GOTFRYD; MARCELO STINSON; MONICA VERMEULEN; JULIETA ALCAIN; SILVIA VANZULLI; MAITE CAMBIASSO; ZAIRA NAGUILA; ALEJANDRA ERLEJMAN; JUAN CARLOS CALVO; GABRIELA SALAMONE; VANINA FONTANA

Ciudad Autónoma de Buenos Aires

Congreso; International Federation of Placenta Associations 2019 (IFPA2019); 2019

IFPA/SLIMP

Previously, we observed that male alcohol consumption delayed embryo differentiation by deregulating peri-implantation events and altering embryo trophoblast and inner cell mass morphology in vitro. Objective: To evaluate the effects of paternal alcohol consumption on the offspring in mice. Methods: CF-1 male mice were exposed (treated group) or not (control group) to 15% (v/v) ethanol in drinking water ad libitum for 15 days. Control and treated males were mated with non-treated CF-1 females (1:1), and pregnant females (vaginal plug: day1) were determined. Pregnancy outcome was evaluated and litter mortality, weight and size registered. Blood from adult offspring was analyzed in a Coulter counter. Then, they were sacrificed and dissected. Offspring´s spleen was weighed and analyzed histologically. Spleen cell populations were determined by flow cytometry. Results: After 21 days of pregnancy, born mice from both groups were registered and, during the first week after birth, an increased number of deaths from treated group (p<0.001) were observed, when compared to control group. We found less activity and poor fur in those pups from treated vs. control groups, in addition to an alteration in offspring?s weight. We performed a complete blood analysis in both groups and observed that platelet count was higher (p<0.05) and lymphocyte count was lower (p<0.05) in treated vs control groups. Besides, spleen CD19 population was increased (p<0.05) and CD8 and CD11b were decreased in treated group vs controls (p<0.05 for both determinations). The spleen histopathological analysis showed a follicular lymphoid hyperplasia of white pulp. Conclusion: The paternal consumption of alcohol for a short period in mice, affects the offspring?s health since we observed an alteration in the weight of the spleen and cell populations, probably causing a decrease in the survival of the litters in the first week of life.