Effects of Tessaria absinthioides aqueous extract in murine melanoma.

SOSA LOCHEDINO AL; PERSIA A; GÓMEZ L; HAPON MB; GAMARRA-LUQUES C

San Luis

Encuentro; XXXVII REUNIÓN CIENTIFICA ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO; 2019

Sociedad de Biología de Cuyo

Effects of Tessaria absinthioides aqueous extract in murine melanoma.Sosa-Lochedino AL1, Persia A1, Gómez L1, Hapon MB1,2, Gamarra-Luques C1,31 Instituto de Medicina y Biología Experimental de Cuyo (IMBECU) ? Universidad Nacional de Cuyo (UNCuyo), CONICET, 2 Facultad de Ciencias Exactas y Naturales ? UNCuyo, 3 Facultad de Ciencias Médicas ? UNCuyo. e-mail: cgamarraluques@gmail.comMelanoma is a very aggressive tumor that rely on the activity of multiple signaling pathways for their growth, survival and propagation, and adapt quickly to new treatments becoming resistant. Mentioned characteristics make melanoma one of the most aggressive and therapy-resistant cancers. Therefore, finding new treatments to improve patient outcomes is an ongoing effort. We previously demonstrated that Tessaria absinthioides aqueous extract (TaAE) displayed cytotoxic effects against several human cancer cell lines (HeLa, Gli-67, HCT-116, MCF-7) and exerted antitumoral effects in rodents with induced colorectal cancer. The goal of the present work is to demonstrate in vitro and in vivo TaAE activity against melanoma. By the use of B16F0 murine melanoma cell line, we evidenced that TaAE induced changes in proliferation using MTT assay, in a dose-response experimental design, for 48 h. In vitro, median dose (Dm), defined as the dose producing a response of 50 percent, was calculated with Compusyn Software to estimate treatment potency. Moreover, the same cell line was subcutaneously inoculated in C57BL6/wt mice to prove the antitumoral effects of TaAE, orally administrated, at doses of 150 mg/animal/day, for 22 days. Carboplatin (Cbp) was used as chemotherapic treatment control. In culture, TaAE treatment induce a dose-dependent reduction in proliferation, calculated Dm was 14.25 µg/ml (Cbp Dm = 73.19 µg/ml). In vivo, oral treatment induce a delayed tumor detection (Mean = 14 days), reduce tumoral volume doubling time (3.09 days) and diminish the final tumor volume (2.19 cm3). Always, mice TaAE treated evidence improved values in relation to untreated (Mean = 12 days; DT = 2.96 days; Vol = 4.75 cm3) and Cbp treated animals (Mean = 13 days; DT = 2.36 days; Vol = 3.55 cm3). Resuming, TaAE demonstrate in vitro and vivo anti-melanoma effects. Moreover, measured effects in subcutaneous tumors evidence the capability of compounds present in the aqueous extract to be absorbed in the bdigestive system and to circulate to exert systemic antitumoral activity. Together, obtained results support the research of TaAE as a natural compound effective in cancer treatment. Further studies need to describe the cellular and molecular intermediaries of mentioned effects.