Long Read Sequencing (3G) Technologies: Challenges and Opportunities in Plant Genomics

TAPIA, E.

Rafaela

Jornada; III JORNADAS REGIONALES DE GENETICA DEL LITORAL; 2018

Sociedad Argentina de Genética - Filial Litoral

We are now facing a new wave of astonishing advances in high throughput sequencingtechnologies. In this new wave, second-generation (2G) short-read sequencing technologies arequestioned for the first time concerning their capacity for uncovering the secrets of highlyrepetitive genomic regions like those arising in plant genomes. In this presentation, we willdiscuss the challenges and opportunities offered by third-generation (3G) long-read sequencingtechnologies concerning target sequencing applications in, but not limited to, plant sciences.Motivated by the affordable cost of the initial capital investment, we will focus on Nanoporesequencing technologies, exploring the power and beauty of truly long but extremely noisyNanopore reads, the difficulties of highly multiplexed wet-lab protocols, and challenging issuesin bioinformatics data analysis.