CYTOTOXICITY, GENOTOXICITY AND PROLIFERATION EVALUATION OF 3T3-L1 CELL CULTURES IN CONTACT WITH POLY(N-ISOPROPYL ACRYLAMIDE) HYDROGELS

CAPELLA V.; RIVERO R.; LIAUDAT A; ROMA D.; IBARRA L.; ALUSTIZA F.; OLMOS NICOTRA M.F.; BARBERO C.; MAÑAS F.; BOSCH P.; RIVAROLA C.R.; RODRIGUEZ N.

Tafí del Valle - Tucuman

Congreso; XXXIII Jornadas Científicas de la Asociación de Biología de Tucuman; 2016

Asociacion de Biologia de Tucuman

Hydrogels are crosslinked polymers with high affinity for aqueous solutions that have shown great potential for biomedicalapplications. The aim of the present research was to evaluate cytotoxicity, genotoxicity and proliferation of the 3T3-L1 cellline exposed to PNIPAM hydrogel. The hydrogel used was synthesized via free radical polymerization of the monomer Nisopropylacrylamide(NIPAM). Cellular toxicity of cultures exposed for 96 hours to hydrogel was evaluated by 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT), neutral red and trypan blue. In addition, genotoxicity wasassessed using the single-cell electrophoresis assay (comet assay) after 4 days of contact. Finally, cell proliferation wasstudied by incorporation of tritiated thymidine (H3) in the 3T3-L1 cells exposed for 24 and 48 hours to hydrogel. Nosignificant differences were found in cytotoxicity and genotoxicity assays between cells exposed to the hydrogel and thosenot exposed to it. Cell proliferation was not affected by the presence of the hydrogel. These preliminary results indicate thatthe PNIPAM hydrogel is a candidate for use as a surface for cell culture in 2D and for future applications in the field ofregenerative medicine and tissue engineering.