INVESTIGADORES
REMES LENICOV Federico
congresos y reuniones científicas
Título:
Increased LPS-induced production of TNF by monocytes after long-term exposure to prostaglandin E2 is mediated by homologous receptor desensitization
Autor/es:
F. DI DIEGO GARCÍA; A. PALETTA; A. VARESE; J. GEFFNER; A. CEBALLOS; F. REMES LENICOV
Lugar:
Mar del Plata
Reunión:
Congreso; LXVI REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGIA; 2018
Institución organizadora:
Sociedad Argentina de Microbiología
Resumen:
Prostaglandin E2 (PGE2) is a pleiotropic agent produced during acute and chronic inflammation. Presence of PGE2 during LPS stimulation of monocytes results in the inhibition of LPS-induced production of TNF. Previously, we have shown that long-term exposure of monocytes to PGE2 resulted in an increased ability to produce LPS-induced TNF. Here, we analyzed the mechanism of this paradoxical effect.Monocytes were purified from peripheral blood using CD14+ magnetic microbeads. Monocytes were cultured for 15 hs in the presence of PGE2 (10-8M), or not (control), and then stimulated with LPS (25 ng/ml). Production of TNF was evaluated by ELISA or intracytoplasmic staining and flow cytometry.Monocytes pre-treated for 15 hs with PGE2 showed an increase in the production of TNF compared to control cultures: 75 +/- 8 vs 56 +/- 7% TNF+ cells (n=9, p
