Glycoconjugates in branchial mucous cells of Corydoras paleatus Jenyns.

PLAUL SE; ANDRÉS LAUBE PF; DÍAZ AO; BARBEITO CG

Punta del Este Uruguay

Congreso; Ninth Congress of Vertebrate Morphology 26th to 31st July 2010; 2010

The mucous cells of primary and secondary lamellae have been associated with different functions, such as lubrication, protection, inhibition of microorganisms and regulation and diffusion of ionic metabolism. The Neotropical teleost Corydoras paleatus inhabits in shallow freshwater sources. The aim of this study was to analyze the carbohydrate composition of mucous cells (MC) of this species with hystochemical techniques. We analize adult specimens collected in sources of water near the urban of La Plata and were sent from a local fish farm. Samples were fixed by immersion in Bouin’s fluid and routinely processed and embedded in paraffin wax. Several histochemical methods to identify and differentiate glycoconjugates (GCs) with: 1) oxidizable vicinal diols (PAS); 2) sialic acids (SA) and of their chain variants: C7, C8 o C9 (PAS*S); 3) AS residues (KOH/PA*S); 4) AS residues with O-acyl substitution at C7, C8 or C9 and O-acyl sugars (PA/Bh/KOH/PAS); 5) neutral with oxidizable vicinal diols  KOH/PA*/Bh/PAS); 6) carboxil groups and/or with O-sulphate esters (AB pH 2.5 and AT pH 5.6); 7) sulphate groups (AB pHs 1.0, 0.5 and AT pH 4.2). The presence of different types of GCs has been associated with different functions of MC. This knowledge is necessary to recognize changes of the GCs patterns in Corydoras living in different environmental conditions. mucous cells (MC) of this species with hystochemical techniques. We analize adult specimens collected in sources of water near the urban of La Plata and were sent from a local fish farm. Samples were fixed by immersion in Bouin’s fluid and routinely processed and embedded in paraffin wax. Several histochemical methods to identify and differentiate glycoconjugates (GCs) with: 1) oxidizable vicinal diols (PAS); 2) sialic acids (SA) and of their chain variants: C7, C8 o C9 (PAS*S); 3) AS residues (KOH/PA*S); 4) AS residues with O-acyl substitution at C7, C8 or C9 and O-acyl sugars (PA/Bh/KOH/PAS); 5) neutral with oxidizable vicinal diols  KOH/PA*/Bh/PAS); 6) carboxil groups and/or with O-sulphate esters (AB pH 2.5 and AT pH 5.6); 7) sulphate groups (AB pHs 1.0, 0.5 and AT pH 4.2). The presence of different types of GCs has been associated with different functions of MC. This knowledge is necessary to recognize changes of the GCs patterns in Corydoras living in different environmental conditions. mucous cells (MC) of this species with hystochemical techniques. We analize adult specimens collected in sources of water near the urban of La Plata and were sent from a local fish farm. Samples were fixed by immersion in Bouin’s fluid and routinely processed and embedded in paraffin wax. Several histochemical methods to identify and differentiate glycoconjugates (GCs) with: 1) oxidizable vicinal diols (PAS); 2) sialic acids (SA) and of their chain variants: C7, C8 o C9 (PAS*S); 3) AS residues (KOH/PA*S); 4) AS residues with O-acyl substitution at C7, C8 or C9 and O-acyl sugars (PA/Bh/KOH/PAS); 5) neutral with oxidizable vicinal diols  KOH/PA*/Bh/PAS); 6) carboxil groups and/or with O-sulphate esters (AB pH 2.5 and AT pH 5.6); 7) sulphate groups (AB pHs 1.0, 0.5 and AT pH 4.2). The presence of different types of GCs has been associated with different functions of MC. This knowledge is necessary to recognize changes of the GCs patterns in Corydoras living in different environmental conditions. Corydoras paleatus inhabits in shallow freshwater sources. The aim of this study was to analyze the carbohydrate composition of mucous cells (MC) of this species with hystochemical techniques. We analize adult specimens collected in sources of water near the urban of La Plata and were sent from a local fish farm. Samples were fixed by immersion in Bouin’s fluid and routinely processed and embedded in paraffin wax. Several histochemical methods to identify and differentiate glycoconjugates (GCs) with: 1) oxidizable vicinal diols (PAS); 2) sialic acids (SA) and of their chain variants: C7, C8 o C9 (PAS*S); 3) AS residues (KOH/PA*S); 4) AS residues with O-acyl substitution at C7, C8 or C9 and O-acyl sugars (PA/Bh/KOH/PAS); 5) neutral with oxidizable vicinal diols  KOH/PA*/Bh/PAS); 6) carboxil groups and/or with O-sulphate esters (AB pH 2.5 and AT pH 5.6); 7) sulphate groups (AB pHs 1.0, 0.5 and AT pH 4.2). The presence of different types of GCs has been associated with different functions of MC. This knowledge is necessary to recognize changes of the GCs patterns in Corydoras living in different environmental conditions.